Specific amplification with PCR of a refractory segment of genomic DNA
نویسندگان
چکیده
منابع مشابه
SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
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ABSTRACT Recently, PCR is being reported more frequently with satisfactory results on the diagnosis of clinical infections. Widespread availability of PCR promises a sensitive and specific alternative, to traditional methods, but these benefits must be balanced against cost. Protocols using small volumes have described where the reaction taken place in capillary tubes, and small volumes work...
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BACKGROUND Despite the rapid transition into routine clinical practice of molecular techniques based on PCR, external quality assessment (EQA) is still not widely available. The European Union and European Communities Confederation of Clinical Chemistry have supported the EQUAL project as a series of 3 different EQA programs for the assessment of molecular methods independently from analytes. W...
متن کاملspecific amplification of aspergillus fumlgatus dna by polymerase chain reaction
invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. an early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. recently, the polymerase chain reaction (pcr) has been used successfully in detecting specific dna of several pathogen. in this study, nested pcr was used to detect dna specific for a!.pergiflus s...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.24.11844